قلم IHC، قلم داکو، Dako pen، پاپ پن، داکوپن، Pap pen
ساخت کمپانی Daido Sangyo ژاپن
قلم IHC که با نام های داکو پن، قلم داکو، Dako pen، پاپ پن، Pap pen، نیز شناخته می شود به منظور جلوگیری از هدر رفتن آنتی بادی های گرانقیمت در رنگ آمیزی بافت در روش های ایمونوهیستوشیمی، ایمونوسیتوشیمی و ایمونوفلورسانس استفاده می شود.
اساس عملکرد آن بر مبنای خواص ترکیبات هیدروفوبیک است. به این منظور یک لایه از آن را بر روی لام و در اطراف نمونه ایجاد می کند که باعث می شود محلولهای ریخته شده بر روی نمونه به اطراف راه پیدا نکند و از مرز ایجاد شده در اطراف آنها توسط قلم IHC عبور نکنند به همین دلیل به آن hydrophobıc barrier pen نیز گفته می شود.
• آماده برای استفاده
• قابلیت استفاده حتی بر روی سطح لام خیس
• قابلیت تحمل دما تا 120 درجه سانتیگراد
• غیر قابل انحلال در الکل و استون
• قابل استفاده برای رنگ آمیزی های مایکروویو و in situ hybridization
IHC Pen, PAP Pen
This ready-to-use hydrophobic barrier pen is designed for immunohistochemistry applications. The surface tension provided by the circle, square, or any other shapes drawn to the tissue sections with the pen, ensures that only the amount of antibody solution needed for the sufficient reaction will be applied. The pen is stable up to 129 °C in microwave heat. The PAP Pen is an effective tool for immunostaining procedures by the Peroxidase-Anti peroxidase (PAP) method, ABC method, LSAB method, polymeric method, immunofluorescence method, ASD method, Enzyme method and Frozen Section method. For paraffin sections, the PAP Pen should be applied after deparanization. For frozen sections, the PAP Pen can be applied after fixation.
Liquid Blocker (Super Pap Pen) has an innovative advance in its efficiency, especially on the following point:
Broad usage in immunostaining (PAP method, ABC method) and fluorescent antibody method
Heat resistance – up to 120 °C
Minimizing your immunostaining procedures;
– available to apply directly onto the wet glass slide
– no more slide preparation work necessary such as wiping/drying out the wet glass slide
Securing more accurate and firm adhesion to the glass slide
Liuid Blocker (Super Pap Pen) makes a water repellent circle which avoids the procedure to wipe-out around the section as used to at every staining of antiserum.
Since serum is guarded by the Liquid Blocker circle, it is assured of getting successful work-out in staining.
Circle made by Liquid Blocker is removable by xylene. Since it is not influenced by water-soluble mounting media, there is no interruption for the microscopic observation.
This water repllent Liquid Blocker is also applicable to the microwave staining, in situ hibridization, etc.
The PAP-PEN Liquid Blocker pen is suitable for immunohistochemical staining (PAP method, ABC method, etc.) And the fluorescent antibody method.
The pen deposits a hydrophobic waxy film useful for defining the coloring area.
And insoluble in alcohol and in acetone. It is not influenced by temperature.
Before use, hold the pen upright and press lightly preventing the liquid leak excessively, but making sure to drain into the tip.
PAP PEN Liquid Blocker supports the following advantages over a traditional pen for immunohistochemistry:
– Write on wet glass;
– The waxy film resists to 120 ° C;
– It is also usable with colors that include the use of microwaves, and in situ hybridization.
MATERIAL USED: metal body, plastic cap, felt tip
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